A Reid 1, Peter A Mollica 2, Robert D Bruno 3, Patrick C Sachs 4
Fig. 4 Bioprinting of non-linear organoids. MCF12A cells were printed in 40-cell clusters in a radial pattern with print spacings of (a) 500 μm, (b) 400 μm, and (c) 300 μm. (a1–c1) Image of printed cell clusters 7 days after print demonstrating early development into individual organoids. (a2–c2) Image of printed cell clusters 14 days after print demonstrating fusion of individual organoids into a contiguous circular organoid. (d) Image of red fluorescent protein–positive (RFP+) MCF12A cells forming large circular organoid 14 days after print. (e) Example of a large circular organoid 24 days after print measuring about 4 mm in diameter. (f) Hematoxylin and eosin (H&E) cross-section of circular organoid shown in e demonstrating luminal sections within the organoid. Scale bars = 500 μm
https://pubmed.ncbi.nlm.nih.gov/30305139/
Reid, J.A., Mollica, P.A., Bruno, R.D. et al. Consistent and reproducible cultures of large-scale 3D mammary epithelial structures using an accessible bioprinting platform. Breast Cancer Res 20, 122 (2018). https://doi.org/10.1186/s13058-018-1045-4